Shobhan Gaddameedhi, Ph.D.

Education & Fellowships
Postdoctoral fellow, University of North Carolina School of Medicine, Chapel Hill, N.C., 2008-14, Mentor: Professor Aziz Sancar, NAS member, Nobel Laureate

Ph.D. in pharmaceutical sciences, North Dakota State University, Fargo, N.D., 2008, Mentor: Dr. Satadal Chatterjee

Master of Science in plant sciences, University of Hyderabad, Hyderabad, India, 2001

Bachelor of Science in biology and chemistry, Osmania University, Hyderabad, India, 1999

Additional Training
Graduate summer intern, hematology/oncology division, Amgen, Thousand Oaks, Calif., 2007, Mentors: Drs. Angus Sinclair and Liqin Liu

Graduate research assistant in biological sciences, North Dakota State University, 2002-03, Mentor: Dr. Marc D. Anderson

Research assistant in microbiology & cell biology, Indian Institute of Science (IISc), Bangalore, India, 2002, Mentor: Professor Lakshmi Sita

Research intern, International Crops Research Institute for Semi-Arid Tropics (ICRISAT), Hyderabad, India, 2001, Mentor: Dr. Kiran K. Sharma

Honors & Awards
Graduate Program Teacher of the Year Award from WSU College of Pharmacy, 2017

EMGS Young Scientist Award Nomination, 2017

NIH Pathway Independent Award (K99/R00), 2013

UNC Postdoctoral Award for Research Excellence, 2013

Travel award to attend Young Investigator Meeting in India, 2013

Third place poster award from the Oliver Smithies Nobel Symposium, 2012

EMGS New Investigator Travel Award, 2011

PRACS Institute Graduate Student Research Excellence Award, 2006

Merit Scholarship Award for being a top ranker (all India level) on the “entrance examination” at the University of Hyderabad, 1999

Research
Every cell in the body has a circadian clock that regulates the biochemical and cellular processes such as sleep, body temperature, metabolism, hormone levels, physiology and behavior of an organism. Mouse models suggest that up to 43% of protein coding genes display circadian rhythmicity at the transcriptional level. Disruption of circadian rhythms can occur through shift work or jet lag, and a strong correlation between circadian disruption and several cancer types has been demonstrated through small animal experimental studies and human epidemiological studies. There is still, however, a lack of evidence at the cellular and molecular levels for humans, which the Gaddameedhi Lab addresses through the following key questions:

What are the potential molecular mechanisms regulating environmental carcinogenesis via circadian disruption by long-term shift work/chronic jet lag in humans?

What are the molecular interactions between the circadian clock and solar UV-B radiation, how does that interaction impact skin disease outcomes such as photoaging and skin cancers, and how do ‘gene-environment’ interactions mediate those outcomes?

What is the molecular basis of the circadian clock's role in the improved efficacy of radiation treatment mediated cancer therapeutics through chronoradiotherapy (CRT)?

Our long-term goal is to translate the general principles of circadian clock that govern environmental carcinogenesis prevention and its impact on improving the therapeutic efficacies of current anti-cancer modalities. We are addressing these key queries using a combination of various innovative and multifaceted approaches including real-time animal bioluminescence imaging to monitor progressive tumor growth and metastasis noninvasively, luciferase bioluminescence reporters to monitor cell-autonomous circadian phase shift, multiplex gene expression arrays, chromatin immunoprecipitation, quantitative immuno-slot blot and western blot analysis, X-Rad 320 irradiator with precise radiation treatment dosage, genetically engineered mouse models (GEMMs), and the circadian synchronized and shiftwork simulated human tissue biopsies.

Selected Publications
James SM, Honn KA, Gaddameedhi S and Van Dongen HPA (2017): Shift Work: Disrupted Circadian Rhythms and Sleep Implications for Health and Well-being, Curr Sleep Medicine Rep., 3(2):104-112

Dakup P and Gaddameedhi S* (2017): Impact of the Circadian Clock on UV-Induced DNA Damage Response and Photocarcinogenesis, Photochem. Photobiol., 93:296-303, *Corresponding author

Gajula RP and Gaddameedhi S* (2015): Commentary: Chemiexcitation of melanin derivatives induces DNA photoproducts long after UV exposure, Front Physiol., 6:276. *Corresponding author

Manjanatha MG, Shelton SD, Chen Y, Gaddameedhi S, Howard PC, Boudreau MD (2015): Development and validation of a new transgenic hairless albino mouse as a mutational model for potential assessment of photocarcinogenicity, Mutat Res., 791: 42-52

Simpson DA, Lemonie N, Morgan DS, Gaddameedhi S, and Kaufmann WK (2015): Oncogenic BRAF (V600E) Induced Clastogenesis and UVB Hypersensitivity, Cancers, 7: 1072-1090

Gaddameedhi S*, Selby CP, Kemp MG, Ye R, and Sancar A* (2015): The Circadian Clock Controls Sunburn Apoptosis and Erythema in Mouse Skin, J. Invest. Dermatol., 135: 1119- 1127. *Corresponding authors

Sancar A, Lindsey-Boltz LA, Gaddameedhi S, Selby CP, Ye R, Chiou YY, Kemp MG, Hu J, Lee JH, Ozturk N (2015): Circadian Clock, Cancer, and Chemotherapy, Biochemistry, 54(2): 110-123

Ye R, Selby CP, Chiou Y, Dagliyan I, Gaddameedhi S, and Sancar A (2014): Dual modes of CLOCK:BMAL1 inhibition mediated by Cryptochrome and Period proteins in the mammalian circadian clock, Genes Dev., 28:1989-1998

Kemp MG, Gaddameedhi S, Hu J, Choi JH, and Sancar A (2014): DNA Repair Synthesis and Ligation Affect the Processing of Excised Oligonucleotides Generated by Human Nucleotide Excision Repair, J. Biol. Chem., 289 (38): 26574-26583

Choi JH, Gaddameedhi S, Hu J, Kemp MG, and Sancar A (2014): Highly specific and sensitive method for measuring nucleotide excision repair kinetics of UV photoproducts in human cells, Nucleic Acids Res., 42(4), e29

Hu J, Choi JH, Gaddameedhi S, Kemp MG, Reardon JT, and Sancar A (2013): Nucleotide Excision Repair in Human Cells: Fate of the Excised Oligonucleotide Carrying DNA Damage In Vivo. J. Biol. Chem., 288 (29): 20918-20926

Lee JH, Gaddameedhi S, Ozturk N, Ye R and Sancar A (2013): DNA damage-specific control of cell death by cryptochrome in p53 mutant Ras-transformed cells. Cancer Res., 73 (2): 785-791

Gaddameedhi S, Reardon JT, Ye R, Ozturk N and Sancar A (2012): Effect of circadian clock mutations on DNA damage response in mammalian cells. Cell Cycle, 11(18): 3481-3491. Selected for News & Views by Cell Cycle

Gaddameedhi S, Selby CP, Kaufmann WK, Smart RC, and Sancar A (2011): Control of Skin Cancer by the Circadian Rhythm. Proc Natl Acad Sci USA, 108(46): 18790-18795. Selected for commentary and front page highlight by PNAS. Selected by Faculty of 1000 Biology twice by two independent investigators

Gaddameedhi S and Sancar A (2011): Melanoma and DNA Damage from a Distance (Farstander Effect). Pigment Cell Melanoma Res., 24: 3-4

Gaddameedhi S, Kemp MG, Reardon JT, Shields J, Smith-Roe SL, Kaufmann WK, and Sancar A (2010): Similar Nucleotide Excision Repair Capacity in Melanocytes and Melanoma Cells. Cancer Res., 70(12):4922-4930

Ozturk N, Lee JH, Gaddameedhi S and Sancar A (2009): Loss of cryptochrome reduces cancer risk in p53 mutant mice. Proc Natl Acad Sci USA, 106 (8): 2841-2846. Selected by Faculty of 1000 Biology

Gaddameedhi S and Chatterjee S (2009): Association between Unfolded Protein Response, induced by 2-deoxyglucose, and hypersensitivity to cisplatin: A mechanistic study employing molecular genomics. J. Cancer Res. Ther., 5(9): 61-66

Book Chapters
Cho K, Gajula R and Gaddameedhi S* (2016): The Cutaneous Circadian Clock as a Determinant of Environmental Vulnerability: Molecular Pathways and Therapeutic Opportunities, Skin Stress Response Pathways, Springer International Biomedical Sciences, 415-432. *Corresponding author

Chatterjee S and Gaddameedhi S (2011): In-Depth Look: anti-TNF-α Therapies, Development of Therapeutic Agents Handbook, John Wiley & Sons, 1045-1092

Funding Support (current and past funding as a PI)
NIH (R00) Award, January 2015- December 2017

WSU College of Pharmacy Start-up, September 2014- August 2018

NIH K99 Award, September 2013- August 2014

Pilot Project Grant Award from the Center for Environmental Health and Susceptibility (CEHS) at UNC, 2013- 2014

Pilot Grant Award from the North Carolina Translational and Clinical Sciences (NC TraCS) Institute, 2012-2013

Pilot Project Grant Award from the CEHS at UNC, 2011- 2012

Invited Talks
Smerdon/Reeves Chromatin-DNA Repair Lecture, Pullman, Wash., 2017

Radiation Research Society (RRS) Annual Meeting, Waikoloa Village, Hawaii, 2016

American Society for Photobiology (ASP) Annual Meeting, Tampa, Fla., 2016

ASPET 2016 Annual Meeting mini symposium on “Chronopharmacology in Cancer: Does Time Really Matter?”, San Diego, Calif., 2016

AAPS RMDG Annual Meeting on “Pharmaceutical Sciences: From Discovery to Translation”, Breckenridge, Colo., 2015

University of Washington Dermatology Division, Seattle, Wash., invited talk, 2015

Oregon Institute of Occupational Health Sciences, Portland, Ore., invited talk, 2015

AACR International Conference on Frontiers in Cancer Prevention Research, New Orleans, La., 2014

Fred Hutchinson Cancer Research Center, Seattle, Wash., 2014

NIH-DNA Repair Video Conference Young Investigator talk, 2012

Service
Gaddameedhi currently serves as a chair for the Pharm.D. Honors Program Committee and the Vivarium Committee.

Ad hoc reviewer for the NIH Study Section (CE), 2017

Ad hoc reviewer for the Wellcome Trust of UK collaborative research grants, 2017

Ad hoc reviewer for the NIH Study Section (ACTS), 2016

Ad hoc reviewer for the American Cancer Society (ACS) Scholar in Training Grant Award, 2014

Ad hoc reviewer for the Medical Research Council (MRC) of UK Research Grant Award, 2014

Ad hoc reviewer for the Hungarian Scientific Research Fund (OTKA), 2014

Gaddameedhi also serves as a reviewer for the following journals: Archives of Toxicology, Biochemistry, Cancer Research, Cellular Physiology & Biochemistry Journal, Chemical Research in Toxicology, Chronobiology International, Environmental and Molecular Mutagenesis, Frontiers in Pharmacology, Journal of Investigative Dermatology, Melanoma Research, Oncotarget, Photochemistry and Photobiology, Plos ONE, Skin Pharmacology and Physiology, and Toxicology Reports.

Teaching
Gaddameedhi currently mentors 3 students (2 Ph.D. students and 1 Pharm.D. honors student) and 1 research technologist. In addition to his responsibilities of mentoring the next generation of biomedical research scientists, his classroom teaching focuses on ‘integrated pharmacology’ 4-credit course (as a participating instructor) to Pharm.D. students and ‘fundamentals of chronopharmacology’ 3-credit course (as a founding director and the instructor of record) to Pharm.D. professional and Ph.D. graduate curriculum students.

Mentoring certificate award from UNC for successfully completing 6-week workshop on “Summer Teaching and Pedagogy Series," 2013

Teaching certificate award from UNC for successfully completing 8-week workshop on “Effective Mentoring in the Research Laboratory," 2011

Graduate teaching assistant in NDSU College of Pharmacy for ‘pharmacodynamics/applied therapeutics' course to Pharm. D. professional students between 2006 to 2007

updated 08/15/2017     Back to top
Dr. Shobhan Gaddameedhi

Assistant Professor
Pharmaceutical Sciences

shobhan.gaddameedhi@wsu.edu

Office: PBS 317
P.O. Box 1495
Washington State University
Spokane, WA 99210-1495