Education & Fellowships
Postdoctoral fellow, University of North Carolina School of Medicine, Chapel Hill, N.C., 2008-14
Mentor: Professor Aziz Sancar, NAS member, Nobel Laureate
Ph.D. in pharmaceutical sciences, North Dakota State University, Fargo, N.D., 2008
Master of Science in plant sciences, University of Hyderabad, Hyderabad, India, 2001
Bachelor of Science in biology and chemistry, Osmania University, Hyderabad, India, 1999
Graduate summer intern, hematology/oncology division, Amgen, Thousand Oaks, Calif., 2007
Graduate research assistant in biological sciences, North Dakota State University, 2002-03
Research assistant in microbiology & cell biology, Indian Institute of Science (IISc), Bangalore, India, 2002
Research intern, International Crops Research Institute for Semi-Arid Tropics (ICRISAT), Hyderabad, India, 2001
Honors & Awards
Special invitation from the President of AACR to re-join the organization as a full member, 2014
NIH Pathway Independent Award (K99/R00), 2013
UNC Postdoctoral Award for Research Excellence, 2013
Travel award to attend Young Investigator Meeting in India, 2013
Third place poster award from the Oliver Smithies Nobel Symposium, 2012
EMS New Investigator Travel Award, 2011
PRACS Institute Graduate Student Research Excellence Award, 2006
Merit Scholarship Award for being a top ranker on the “entrance examination” at the University of Hyderabad, 1999
Every cell in the body has a circadian clock that regulates the biochemical and cellular processes such as sleep, body temperature, metabolism, hormone levels, physiology and behavior of an organism. Mouse models suggest that 43% of protein coding genes display circadian rhythmicity at the transcriptional level. Among these, more than 170 drug targets are the clock controlled genes, including 56 of the top 100 best-selling drugs in U.S. There is a high correlation between advent of circadian disruption through shift work to several cancer types as per the data accumulated from experimental studies on small animals and epidemiological studies performed on humans. However, no concrete evidence at cellular and molecular levels are reported for humans per se. In this regard the Gaddameedhi research group addresses these key fundamental questions:
1. To identify the potential molecular biomarkers/pathways regulating carcinogenesis via circadian disruption by shift work in humans.
2. To understand the effect of circadian clock on skin biology via environmental stresses associated with dermatological perturbations including skin cancer.
3. To unravel the molecular basis for chronopharmacology role in improved efficacy of genotoxic stress mediated cancer therapeutics.
The long-term goal is to translate the general principles of circadian clock that govern cancer prevention and its impact on improving the therapeutic efficacies of current anti-cancer modalities. They are addressing these key queries using a combination of various innovative and multifaceted approaches including real-time animal bioluminescence imaging to monitor progressive tumor growth and metastasis noninvasively, luciferase bioluminescence reporters to monitor cell-autonomous circadian phase shift, multiplex gene expression arrays, chromatin immunoprecipitation, quantitative immuno-slot blot and western blot analysis, ionizing radiation treatment using advanced X-Rad 320 irradiator with precise radiation dosage, genetically engineered mouse models (GEMMs), and the circadian synchronized human tissue biopsies.
Gajula RP and Gaddameedhi S* (2015): Commentary: The Protective Role of Melanin on UVR Induced Melanoma: Friend or Foe?, Frontiers in Physiology, 6:1-3 *corresponding author.
Manjanatha MG, Shelton SD, Chen Y, Gaddameedhi S, Howard PC, Boudreau MD (2015): Development and validation of a new transgenic hairless albino mouse as a mutational model for potential assessment of photocarcinogenicity, Mutation Research, 791: 42-52.
Simpson DA, Lemonie N, Morgan DS, Gaddameedhi S, and Kaufmann WK (2015): Oncogenic BRAF (V600E) Induced Clastogenesis and UVB Hypersensitivity, Cancers, 7: 1072-1090.
Gaddameedhi S*, Selby CP, Kemp MG, Ye R, and Sancar A* (2015): The Circadian Clock Controls Sunburn Apoptosis and Erythema in Mouse Skin, Journal of Investigative Dermatology, 135: 1119-1127. *co-corresponding authors.
Sancar A, Lindsey-Boltz LA, Gaddameedhi S, Selby CP, Ye R, Chiou YY, Kemp MG, Hu J, Lee JH, Ozturk N (2015): Circadian Clock, Cancer, and Chemotherapy, Biochemistry, 54(2): 110-123.
Ye R, Selby CP, Chiou Y, Dagliyan I, Gaddameedhi S, and Sancar A (2014): Dual modes of CLOCK:BMAL1 inhibition mediated by Cryptochrome and Period proteins in the mammalian circadian clock, Genes & Development, 28:1989-1998.
Kemp MG, Gaddameedhi S, Hu J, Choi JH, and Sancar A (2014): DNA Repair Synthesis and Ligation Affect the Processing of Excised Oligonucleotides Generated by Human Nucleotide Excision Repair, The Journal of Biological Chemistry, 289(38): 26574-83.
Choi JH, Gaddameedhi S, Hu J, Kemp MG, and Sancar A (2014): Highly specific and sensitive method for measuring nucleotide excision repair kinetics of UV photoproducts in human cells, Nucleic Acids Research, 42 (4), E29 (1-11).
Hu J, Choi JH, Gaddameedhi S, Kemp MG, Reardon JT, and Sancar A (2013): Nucleotide Excision Repair in Human Cells: Fate of the Excised Oligonucleotide Carrying DNA Damage In Vivo. The Journal of Biological Chemistry, 288: 20918-20926.
Lee JH, Gaddameedhi S, Ozturk N, Ye R and Sancar A (2013): DNA damage-specific control of cell death by cryptochrome in p53 mutant Ras-transformed cells. Cancer Research, 73 (2): 785-791.
Gaddameedhi S, Reardon JT, Ye R, Ozturk N and Sancar A (2012): Effect of circadian clock mutations on DNA damage response in mammalian cells. Cell Cycle, 11(18): 3481-3491.
Selected for News & Views by Cell Cycle
Gaddameedhi S, Selby CP, Kaufmann WK, Smart RC, and Sancar A (2011): Control of Skin Cancer by the Circadian Rhythm. Proc Natl Acad Sci USA, 108 (46): 18790-18795.
Selected for commentary and front page highlight by PNAS
Selected by Faculty of 1000 Biology twice by two independent investigators
Gaddameedhi S and Sancar A (2011): Melanoma and DNA Damage from a Distance (Farstander Effect). Pigment Cell & Melanoma Research, 24: 3-4.
Gaddameedhi S, Kemp MG, Reardon JT, Shields J, Smith-Roe SL, Kaufmann WK, and Sancar A (2010): Similar Nucleotide Excision Repair Capacity in Melanocytes and Melanoma Cells. Cancer Research, 70(12):4922-4930.
Ozturk N, Lee JH, Gaddameedhi S and Sancar A (2009): Loss of cryptochrome reduces cancer risk in p53 mutant mice. Proc Natl Acad Sci USA, 106 (8): 2841-2846.
Selected by Faculty of 1000 Biology
Gaddameedhi S and Chatterjee S (2009): Association between Unfolded Protein Response, induced by 2-deoxyglucose, and hypersensitivity to cisplatin: A mechanistic study employing molecular genomics. Journal of Cancer Research and Therapeutics, 5(9): 61-66.
Ana D. Simonovic, Gaddameedhi S and Marc D. Anderson (2004): In-gel precipitation of enzymatically released phosphate. Analytical Biochemistry, 334: 312-317.
Chatterjee S and Gaddameedhi S (2011): In-Depth Look: anti-TNF-α Therapies, Pages 1045-1092. (Title of the Book: Development of Therapeutic Agents Handbook, Edited by Gad Shayne Cox, ISBN-13: 978-0-471-21385-7 - John Wiley & Sons)
Funding Support (current and past funding as a PI)
NIH (R00) Award, January 2015- December 2017
WSU College of Pharmacy Start-up, September 2014- August 2018
NIH K99 Award, September 2013- August 2014
Pilot Project Grant Award (NIH-398 format) from the Center for Environmental Health and Susceptibility (CEHS) at UNC, 2013- 2014
NC TraCS $2K Grant Award from the North Carolina Translational and Clinical Sciences (NC TraCS) Institute, 2012-2013
Pilot Project Grant Award (NIH-398 format) from the CEHS at UNC, 2011- 2012
AAPS RMDG Annual Meeting on “Pharmaceutical Sciences: From Discovery to Translation”, Breckenridge, Colo., 2015
University of Washington Dermatology Division, Seattle, Wash., invited talk, 2015
Oregon Institute of Occupational Health Sciences, Portland, Ore., invited talk, 2015
AACR International Conference on Frontiers in Cancer Prevention Research, New Orleans, La., 2014
Fred Hutchinson Cancer Research Center, Seattle, Wash., 2014
NIH-DNA Repair Video Conference Young Investigator talk, 2012
International Max Planck Research School, Cologne, Germany, 2002
Gaddameedhi currently serves as a chair for the Pharm.D. honors program committee and as a member for the graduate program committee for the WSU College of Pharmacy.
Ad hoc reviewer for the American Cancer Society (ACS) Scholar in Training Grant Award, 2014
Ad hoc reviewer for the Medical Research Council (MRC) of UK Research Grant Award, 2014
Ad hoc reviewer for the Hungarian Scientific Research Fund (OTKA), 2014
Gaddameedhi also serves as a reviewer for the following journals: Archives of Toxicology, Biochemistry, Cellular Physiology & Biochemistry Journal, Chemical Research in Toxicology, Chronobiology International, Frontiers in Pharmacology, Melanoma Research, Oncotarget, Pharmaceutical Biology, Plos ONE, and Toxicology Reports.
Participant in Cutaneous Oncology and the Road to Personalized Care at UNC organized by UNC Lineberger Cancer Center on February 29, 2012 to synthesize research ideas to develop future research platform in the field of oncology.
Participant in UNC summer undergraduate student research program (delivered research presentation, conducted journal club meeting, and served as a judge in their poster presentation) in summer 2011.
Gaddameedhi currently mentors 3 students (1 graduate student, 1 Pharm.D. honors student and 1 high school student), 2 post-doctoral fellows and 1 research intern. In addition to his responsibilities of mentoring the next generation of biomedical research scientists, his classroom teaching focuses on integrated pharmacology to Pharm.D. students and fundamentals of chronopharmacology to Pharm.D. professional and Ph.D. graduate curriculum students.
Mentoring certificate award from the UNC for successfully completing 6-week workshop on “Summer Teaching and Pedagogy Series," 2013
Teaching certificate award from the UNC for successfully completing 8-week workshop on “Effective Mentoring in the Research Laboratory," 2011
Graduate teaching assistant in NDSU College of Pharmacy for ‘pharmacodynamics/applied therapeutics course’ between 2006 to 2007